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Proteins are major targets of Pharmaceuticals, and are themselves increasingly used as therapeuticals. However both basic research and the pharmaceutical industry depends on availability of purified proteins that are often difficult to isolate from native sources. In this lecture course, students will learn basic and advanced theoretical background in expression and purification of recombinant proteins. It will cover a variety of expression systems, including prokaryotic and eukaryotic cells. The course will also address traditional and new methods in recombinant protein purification. Furthermore, students will be introduced to some downstream applications such as crystallization screens and biochemical/biophysical studies. Student will choose a term project for oral and written presentation.