51视频

BIOL.5290 Recombinant Protein Production Techniques (Formerly 81.429 & 81.529)

Description

This course introduces students to the principles and practice of recombinant protein expression and purification's. Proteins are major targets of pharmaceuticals, and are themselves increasingly used as therapeuticals. However both basic research and pharmaceutical industry depends on availability of purified proteins that are often difficult to isolate from native sources. This course will provide both didactic and laboratory instruction. It is comprised of a series of lecture and laboratory exercises, with an emphasis on practical techniques and hands-on experience of recombinant protein purification. The course will cover a variety of expression systems, including prokaryotic and eukaryotic cells, and address traditional and new methods in protein purification.

Course prerequisites/corequisites are determined by the faculty and approved by the curriculum committees. Students are required to fulfill these requirements prior to enrollment. For courses offered through online or GPS delivery, students are responsible for confirming with the instructor or department that all enrollment requirements have been satisfied before registering.